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Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with <t>DAPI;</t> bottom: cortical areas stained <t>with</t> <t>NT640.</t> e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.
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Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with <t>DAPI;</t> bottom: cortical areas stained <t>with</t> <t>NT640.</t> e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.
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Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with <t>DAPI;</t> bottom: cortical areas stained <t>with</t> <t>NT640.</t> e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.
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Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with <t>DAPI;</t> bottom: cortical areas stained <t>with</t> <t>NT640.</t> e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.
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Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with <t>DAPI;</t> bottom: cortical areas stained <t>with</t> <t>NT640.</t> e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.
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Image Search Results


Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with DAPI; bottom: cortical areas stained with NT640. e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.

Journal: Nature biotechnology

Article Title: High-throughput mapping of a whole rhesus monkey brain at micrometer resolution.

doi: 10.1038/s41587-021-00986-5

Figure Lengend Snippet: Fig. 1 | The SMART approach for high-throughput mapping of a rhesus macaque brain at micron resolution. a, The SMART pipeline. b, Comparison of macaque brain and mouse brain (inset). c, A macaque brain slice before (left) and after (right, flipped) PuClear treatment. d, Comparison between the SDS- and RIMS-based clearing, and PuClear treatment. Top: WM areas stained with DAPI; bottom: cortical areas stained with NT640. e, The VISoR2 imaging system. Inset: schematic showing the synchronization mechanism between laser scanning and camera readout. f, A schematic for rigid stitching within a single slice (top) and nonrigid stitching between adjacent slices (no. n and n – 1; bottom). g, Maximum intensity projection (MIP) image of a 100-µm virtual section of two adjacent stacks (separately color coded) with merged overlapped regions after stitching. h, MIP of a 50-µm virtual section from four consecutive slices. i,j, Distributions of the errors of intraslice (i) and interslice (j) stitching. k, Stitched neurons (each shown in three z-sections) and axonal branches cut into two adjacent brain slices (separately color coded). l, Reconstructed macaque brain with viral labeling of bilateral SC areas. m, MIP of a 30-µm coronal section indicated with a dashed line in l. n, Magnified view of the boxed region in m; arrowheads indicate neurons colabeled by the virus and NT640. Scale bars: b,c, 10 mm; d,k,n, 50 μm; g,h, 100 μm; l,m, 5 mm.

Article Snippet: The following antibodies and dyes and their dilutions were used in this study: Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (GFAP; no. Z0334, Dako), 1:100, Anti-Tyrosine Hydroxylase (TH) Antibody (no. MAB318, Millipore), 1:500, Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H + L) (no. 715-605- 151, Jackson ImmunoResearch Laboratories), 1:200, Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H + L) (no. 711-545-152, Jackson ImmunoResearch Laboratories), 1:200, NT640 (no. N21483, ThermoFisher), 1:200, DAPI (no. C1006, Beyotime Biotechnology), no dilution.

Techniques: High Throughput Screening Assay, Comparison, Slice Preparation, Staining, Imaging, Labeling, Virus